Maus anti-Human IgE Antikörper (Biotin)

Produktnummer ABIN457503

Kurzübersicht für Maus anti-Human IgE Antikörper (Biotin) (ABIN457503)

Target: IgE

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Klon: BSW17-NBS01

Isotyp: IgG1 kappa

Produktdetails

Reaktivität: Human

Wirt: Maus

Spezifität: Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes

Sensitivität: Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone: SUS-11)

Produktmerkmale: IGHE, chromosome 14q32.33

Aufreinigung: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck

Reinheit: > 85 %, tested by analytical Gel Permeation Chromatography

Sterilität: Sterile filtered

Immunogen: purified human myeloma IgE

Anwendungsinformationen

Applikationshinweise: Sterile filtered solution, does not contain Na-azide. Recommended for functional cellular assays (mast cell / basophil activation).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Konservierungsmittel: Azide free

Handhabung: Aliquot to Avoid repeated freezing and thawing.

Lagerung: 4 °C

Antigendetails

Target: IgE

Hintergrund: The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.